CHP1
Sigma
Imprint® Chromatin Immunoprecipitation Kit
Description
| General description | The Imprint® ChIP Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format. |
| Features and Benefits | • Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market • Sensitive—As few as 10,000 cells required for each ChIP sample • Convenient—Fewest steps of any available ChIP protocol • Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications • Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology |
| Legal Information | Imprint is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| GenomePlex is a registered trademark of Rubicon Genomics, Inc. |
Safety
| Hazard Codes | F,Xn |
| Risk Statements | 11-22-36/37/38-67 |
| Safety Statements | 7-16-24/25-26-36 |
| RIDADR | UN 3316 9/PG 2 |
Components
| Kit component only | IP Wash Buffer solution |
| Antibody Buffer | |
| Nuclei Preparation Buffer | |
| Shearing Buffer | |
| Dilution Buffer | |
| DNA Release Buffer | |
| Reversing Solution | |
| Column Preparation Solution | |
| Wash Solution Concentrate | |
| Binding Solution | |
| Elution Solution | |
| Homogenization Buffer | |
| 8-well Stripcaps | |
| GenElute™ Binding Column G | |
| Microcentrifuge collection tube | |
| Proteinase K from Tritirachium album, for molecular biology, >800 units/mL, buffered aqueous glycerol solution, DNAse, Nickase and RNAse, none detected | |
| Assay Stripwells | |
| Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution | |
| Normal Mouse IgG antibody produced in mouse | |
| Anti-RNA polymerase II from mouse | |
| Forward Control Primer (GAPDH) | |
| Reverse Control Primer (GAPDH) |
Related Products
References
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| Mora-Lopez, f., et al., Transcription of PRDM1, the master regulator for plasma cell differentiation, depends on an SP1/SP3/EGR-1 GC-box. Eur. J. Immunol. 38(8), 2316-2324, (2008) | |
| Cheetham, S., et al., SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2'deoxycytidine to increase SPARC expression and improve therapy response. Br. J. Cancer 98(11), 1810-1819, (2008) | |
| Yamada, N., et al, MUC1 expression is regulated by DNA methylation and histone H3 lysine 9 modification in cancer cells. Cancer Res. 68(8), 2708-2716, (2008) | |
| Ulleras, E., et al., NFAT but not NF- | |
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| Teng, Y., et al., AS1411 alters the localization of a complex containing protein arginine methyltransferase 5 and nucleolin. Cancer Res. 67(21), 10491-10500, (2007) | |
| Sakurai, T., et al., Functional roles of Fli-1, a member of the Ets family of transcription factors, in human breast malignancy. Cancer Sci. 98(11), 1775-1784, (2007) | |
| Mora-Lopez, F., et al., Human BSAP and BLIMP1 conform an autoregulatory feedback loop. Blood 110(9), 3150-3157, (2007) | |
| Gao, S., et al., Cloning and characterization of the rat lysyl oxidase gene promoter: identification of core promoter elements and functional nuclear factor I-binding sites. J. Biol. Chem. 282(35), 25322-25337, (2007) | |
| Venteclef, N., and Delerive, P., Interleukin-1 receptor antagonist induction as an additional mechanism for liver receptor homolog-1 to negatively regulate the hepatic acute phase response. J. Biol. Chem. 282(7), 4393-4399, (2007) | |
| Liu, D.X., and Lobie, P.E., Transcriptional activation of p53 by Pitx1. Cell Death Differ. 14(11), 1893-18907, (2007) |
